FOXO4-DRI 10mg (Cellular Senescence Reagent)⁠

FOXO4-DRI Research Reagent | Targeted Senolytic Interfering Peptide

FOXO4-DRI (FOXO4 D-Retro-Inverso) is a specially modified chimerical peptide composed entirely of D-amino acids in a reversed sequence configuration relative to its native L-parent sequence. This structural design grants the compound remarkable resistance against typical intracellular proteolytic enzymes, significantly extending its biological stability window within target in vitro matrices.

In preclinical immune modulation and tissue longevity assays, FOXO4-DRI acts as a competitive antagonist. By preventing endogenous FOXO4 proteins from cross-linking with the master regulatory protein p53, the peptide triggers the release of p53 into the cytoplasm. This targeted interaction provides researchers with an accurate baseline model for studying cell-cycle regulation, tissue restoration, and the clearance of destructive senescent cells.

Experimental Cellular Repair & Senolytic Mechanics

Preclinical investigation using FOXO4-DRI 10mg primarily monitors several fundamental molecular signaling vectors:

• FOXO4-p53 Interruption: Evaluating how blocking this specific binding pocket changes the location of p53 from the nucleus to mitochondria, driving selective cell programs.
• SASP Attenuation: Measuring changes in the secretion rates of Senescence-Associated Secretory Phenotypes, including pro-inflammatory interleukins (IL-6, IL-1α) and matrix metalloproteinases.
• Mitochondrial Membrane Alterations: Tracking active caspase cascades ($Caspase-3/7$ and $Caspase-9$) downstream of structural changes in outer mitochondrial membranes.

Laboratory Reconstitution & Storage Standards

Despite the structural enhancements offered by its all-D-amino acid composition, strict laboratory storage variables should be enforced:

• Long-Term Solid State Preservation: Maintain un-reconstituted, vacuum-packed vials in a laboratory freezer environment at -20°C. Keep away from bright UV arrays or dynamic temperature cycles.
• Solvent Integration: Allow the vial casing to equilibrate fully to ambient room temperature before removing the seal. Reconstitute slowly using Sterile Bacteriostatic Water or laboratory saline. Swirl the vial gently; avoid hard shaking to shield the tertiary protein fields.
• Liquid Storage Constraints: Keep the active solution refrigerated at 2°C to 8°C post-mixing. For reliable experimental results, use the liquid sample within 14 to 21 days.