AICAR 50mg (HPLC-Verified / Batch COA)⁠

AICAR Research Reagent | Cell-Permeable AMPK Activator

AICAR (5-Aminoimidazole-4-carboxamide ribonucleoside, or Acadesine) is an organic substance and nucleoside analog that functions as a cell-permeable precursor to ZMP, an analog of adenosine monophosphate (AMP). Within active laboratory models, AICAR acts as a direct stimulant of AMP-activated protein kinase (AMPK). Because AMPK serves as the master metabolic regulator of cellular energy homeostasis, this chemical is widely utilized to mimic cellular energy depletion in vitro.

Once taken up by cellular structures via nucleoside transporters, AICAR is intracellularly phosphorylated into ZMP. ZMP binds directly to the $\alpha$-subunit of the AMPK complex without altering raw intracellular ATP, ADP, or AMP levels. This makes it an invaluable asset for separating pure metabolic signaling cascades from general cell stress responses within neurological and vascular assay models.

Batch-Specific HPLC Analysis & Validation

To ensure strict reproducibility and eliminate baseline chemical drift during high-sensitivity assays, our AICAR 50mg stock undergoes absolute analytical confirmation:

• HPLC Spectral Characterization: Every compound batch is subjected to High-Performance Liquid Chromatography to verify chemical purity parameters equal or exceed 99.0%.
• Mass Spectrometry Authentication: Verifies exact molecular weight metrics, confirming structural conformity to ensure zero raw unlinked fragments.
• Batch Certificate of Analysis (COA): Accessible analytical profiling safeguards testing matrices from unexpected batch-to-batch deviations.

Experimental Research Context & Mechanisms

Preclinical exploration utilizing AICAR focuses on mapping several distinct cellular pathways:

• Mitochondrial Biogenesis: Investigating the up-regulation of PGC-1$\alpha$ signaling to measure alterations in structural mitochondrial density under low-nutrient simulation.
• Neuroplasticity & Proliferation: Examining the relationship between AMPK pathway signaling and neural stem cell differentiation within localized hippocampal culture models.
• Glucose Uptake Mechanics: Tracking GLUT4 transporter translocation dynamics into skeletal and smooth cell margins completely independent of regular insulin pathways.

Laboratory Storage & Handling Protocols

To protect the ribonucleoside framework from hydrolytic cleavage or temperature-induced structural decay, maintain standard preservation guidelines:

• Lyophilized Protection: Store the dry, unopened solid vials long-term at -20°C inside a dark, desiccant-monitored laboratory freezer setup to prevent exposure to external ambient humidity.
• Reconstitution Framework: Vials must be allowed to rest until they fully match ambient room temperatures before introducing solvent. Reconstitute using sterile aqueous laboratory solvents or Sterile Bacteriostatic Water.
• Fluid Maintenance: Following fluid introduction, store the working solution strictly between 2°C and 6°C. Protect from repetitive freeze-thaw cycles, mix gently via manual rotation without excessive shaking, and conclude all experimental exposures within 7 to 10 days.